高等学校化学学报2013，Vol.34Issue(3)：551-555,5.DOI:10.7503/cjcu20120687
以聚硫堇为电化学探针的非标记型核酸适配体传感器
Label-free Aptamer Biosensor with Poly(thionine) as an Electrochemical Probe
摘要
Abstract
A lable-free aptamer biosensor was fabricated with the electropolymerized poly (thionine) as a redox electrochemical probe and gold nanoparticles as the immobilization matrix for aptamer. Electrochemical impedance spectroscopy was used to monitor the self-assembly process of the aptamer biosensor. The electrochemical behavior of the aptamer biosensor was studied by the cyclic voltammetry ( CV) and differential pulse voltammetry ( DPV). The biosensor shows a good linear range of 1. 0 pg/mL-500 ng/mL for thrombin, R = 0.998, with a detection limit of 0. 38 pg/mL. The biosensor has simple preparation procedure, high sensitivity and strong anti-interference ability.关键词
聚硫堇/金纳米粒子/核酸适配体/凝血酶/传感器Key words
Poly(thionine)/ Gold nanoparticles/ Aptamer/ Thrombin/ Biosensor分类
化学化工引用本文复制引用
杨绍明,查文玲,李红,孙清,刘斌,郑龙珍..以聚硫堇为电化学探针的非标记型核酸适配体传感器[J].高等学校化学学报,2013,34(3):551-555,5.基金项目
国家自然科学基金(批准号:21065004)、江西省自然科学基金(批准号:2009GQH0022)和江西省教育厅科技项目(批准号:GJJ12304)资助. （批准号:21065004）
