广东牙病防治2013，Vol.21Issue(2)：75-80,6.
丝素蛋白-壳聚糖三维多孔支架材料生物相容性及促成骨性能研究
Biocompatibility and osteogenesis of silk fibroinitosan scaffold
摘要
Abstract
Objective Co-culture of silk fibroin( SF)/chitosan( CS) scaffold and MG-63 osteoblast cells in vitro and investigate biocompatibility and osteogenesis to demonstrate the preliminary feasibility of the bone tissue engineering. Methods Prepared scaffolds with freezing -drying and chemical crosslinking methods, and divided the subjects into 40% SF-60% CS group (experimental group) and 100% CS group (control group). Observed the growth of MG-63 osteoblast cells in the scaffolds using Hoechst fluorescence staining and the biocompatibility of the materials by the way of calculating adhesion rate and proliferation rate. Lastly, explored the osteogenesis by the evaluation of formation mineralization nodules and promoting serection of alkaline phosphatase( ALP) by MG-63 cells. Blank control group only used glass slides to culture. Results The cell adhesion rate, the cell proliferation rate, the promotion of MG-63 cell to secret alkaline phosphatase, the result of mineralization indices including the MG-63 cells union, ground substance secretion and the formation of calcified nodule, all indicated that the group of 40% SF-60% CS surpassed the 100% CS group. Conclusion The 40% SF-60% CS scaffold have good biocompatibility and can promote the biological function of osteoblast, showing wide application prospects as bone tissue engineering in the future.关键词
丝素蛋白/壳聚糖/骨组织工程/生物相容性/成骨细胞Key words
Silk fibroin/ Chitosan/ Bone tissue engineering/ Biocompatibility/ Osteoblasts分类
医药卫生引用本文复制引用
柳磊,曾曙光,石勇,容明灯,高文峰,郭泽鸿,许竞..丝素蛋白-壳聚糖三维多孔支架材料生物相容性及促成骨性能研究[J].广东牙病防治,2013,21(2):75-80,6.基金项目
广东省中医药局科研基金资助项目(20111145) （20111145）
广东省科技计划项目(2011B031300022) （2011B031300022）
