广东医学2013，Vol.34Issue(6)：821-826,6.
CD2AP分子mRNA和蛋白的表达在肾组织及细胞损伤中的作用
The roles of CD2AP molecule mRNA and protein in renal tissues and cell injury.
摘要
Abstract
Objective To investigate the roles of CD2AP molecule mRNA and protein in puromycin - induced nephropathy and glomerular podocyte injury in vitro. Methods 64 male SD rats were randomly divided into nephropathy model group and control group ( n = 32 ), in which puromycin ( PAN, 5 mg/kg ) and saline were given respectively. 8 subjects from each groups were sacrificed at the 2nd , 4th, 6th and 8th week. Mouse podocyte cell line ( MPC5 ) in vitro were divided into stimulate group, in which PAN treatment were given, and control group. The cells were collected 12 h, 24 h and 48 h after culture. Light microscopy and inverted microscopy were applied for observation of renal histopathological and cellular pathological changes. Meanwhile, RT - PCR, Western blot and immunofluorescence staining were used for assessment of the expression and distribution of CD2AP mRNA and protein. Results Glomerular mesangial and extracellular matrix proliferation, and partial or extensive focal segmental sclerosis were observed in nephropathy model group, so was the continuous down - regulation of CD2AP mRNA and protein since the 2nd week. Significant shrinkage of podocytes, including pod retraction or disappeared absence and intercellular junction loss, were observed in simulated group; so was the down - regulation of CD2AP mRNA and protein. Even nuclear, cytoplasm and membrane CD2AP distribution was revealed in control group, while coarse granular CD2AP distribution in nucleus and cytoplasm was revealed in stimulatedgroup 12 h and 24 h after stimulation, and even cytoplasm and membrane absence of CD2AP was found after 48 h. Conclusion CD2AP mRNA and protein are both significantly down - regulated in rat puromycin nephropathy model and cultured mouse podocyte cells, suggesting an important indicator of podocyte injury. These changes of CD2AP expression and distribution may be involved in the molecular mechanism of proteinuria.关键词
嘌呤霉素/CD2AP/肾组织/足细胞Key words
puromycin/ CD2AP/ renal tissue/ podocyte引用本文复制引用
李晓艳,于力,于生友,温捷..CD2AP分子mRNA和蛋白的表达在肾组织及细胞损伤中的作用[J].广东医学,2013,34(6):821-826,6.基金项目
国家自然科学基金面上项目(编号:81273205),广东省科技计划项目(编号:2011B020800002),广州市科技计划项目(编号:2010Y1-C521) （编号:81273205）
