广东药学院学报2013，Vol.29Issue(1)：1-5,5.DOI:10.3969/j.issn.1006-8783.2013.01.001
眼用N-三甲基壳聚糖包衣胰岛素纳米脂质体原位凝胶的研制及体外释药研究
Study on the preparation and drug release in vitro of ocular N-trimethyl chitosa N-coated insulin nanoliposomes-in situ gels
摘要
Abstract
Objective To prepare ocular insulin(INS) -loaded cationic nanoliposomes-in situ gels(ISG) and investigate their related characteristics and drug release profiles in vitro. Methods Insulin nanoliposomes (INSL) were prepared by the reverse evaporation method,then coated by N-trimethyl chitosan (TMC60). The optimal amount of poloxamer407 was selected according to the gelation temperature. A novel membraneless model was used to study the drug release in vitro. Results The mean diameter of INSL-ISG before TMC60 coating was (92. 36 ± 0. 20) nm with the Zeta potential of ( - 19. 50 ± 0. 30) mV. The mean diameter of INSL-ISG after TMC60 coating was (93. 93 ±0. 19) nm with the Zeta potential of (13. 23 ±0. 15) mV. The optimal concentration of poloxamer407 in the formula was 25% with the gelation temperature of 23.9 ℃ and 34 ℃ before and after simulation tear dilution. Both the drug release in vitro and gel dissolution profiles of TMC60-INSL-ISG exhibited the characteristics of zero order kinetics. Conclusion TMC60-INSL-ISG showed the advantages of cationic liposomes and in situ gels, and could delay the drug release in vitro, which provided the basis for the further study on the cornea residence time promotion.关键词
N-三甲基壳聚糖/胰岛素/纳米脂质体/原位凝胶/体外释药Key words
N-trimethyl chitosan/ insulin/ nanoliposomes/ in situ gels/ release in vitro分类
医药卫生引用本文复制引用
何文,周晶晶,郭咸希..眼用N-三甲基壳聚糖包衣胰岛素纳米脂质体原位凝胶的研制及体外释药研究[J].广东药学院学报,2013,29(1):1-5,5.
