神经损伤与功能重建2013，Vol.8Issue(2)：80-87,8.DOI:10.3870/sjsscj.2013.02.002
氧化低密度脂蛋白诱导人脐静脉内皮细胞凋亡中microRNA表达谱分析
Analysis of microRNA Expression Profile in Apoptotic Endothelial Cells Induced by ox-LDL
摘要
Abstract
Objective: To observe the expression of microRNA (miRNA) in human umbilical vein endothelial cells (HUVECs) induced by oxidized low-density lipoprotein (ox-LDL) and to make an initial prediction of target genes regulated by differentially expressed miRNAs. Methods: HUVECs were treated by ox-LDL to establish apoptotic model in vitro. miRNA microarrays were employed to detect the expression profile of miRNA in apoptotic HUVECs, and the microarray results were validated by quantitative real-time PCR. The miRNA-targeted genes were predicted by online-available software. The gene ontology (GO) database and KEGG pathway database were used to determine the functions of these target genes. Results: With the use of a microarray, we found that ox-LDL up-regulated four miRNAs (hsa-miR-142-3p, hsa-miR-365, hsa-let-7c, hsa-miR-1207-3p) as well as down-regulated eleven miRNAs (hsa-miR-32, hsa-miR-589, hsa-miR-18b, hsa-miR-429, hsa-miR-155, hsa-miR-590-5p, hsa-miR-1197, hsa-miR-222, hsa-miR-374a, hsa-miR-33a, hsa-miR-375) in HUVECs. The expression levels of hsa-miR-142-3p, hsa-miR-365, hsa-miR-590-5p and hsa-miR-33a were validated in accordance with the results of real-time PCR (qRT-PCR). The bioinformatics analysis indicated that the potential target genes of these aberrantly expressed miRNAs participate in the regulation of cell proliferation, apoptosis, metabolism and oncology gene expression. Conclusion: miRNA expression profile in apoptotic HUVECs induced by ox-LDL has significantly changes, which may contribute to endothelial dysfunction and development of atherosclerosis.关键词
氧化低密度脂蛋白/人脐静脉内皮细胞/凋亡/microRNA/动脉粥样硬化Key words
oxidized low-density lipoprotein/human umbilical vein endothelial cells/apoptosis/microRNA/atherosclerosis分类
医药卫生引用本文复制引用
秦冰,曹宇泽,肖波,杨欢..氧化低密度脂蛋白诱导人脐静脉内皮细胞凋亡中microRNA表达谱分析[J].神经损伤与功能重建,2013,8(2):80-87,8.基金项目
国家自然科学基金(No.81070962) （No.81070962）
