神经损伤与功能重建2013，Vol.8Issue(2)：103-108,6.DOI:10.3870/sjsscj.2013.02.006
pEGFP/A2M(FP6)转染神经干细胞海马移植治疗APP/PS1双转基因AD小鼠的实验观察
Treatment of APP/PS1 Double Transgenic Mice of Alzheimer ‘s Disease by Transplanting pEGFP/A2M (FP6) Transfected Neural Stem Cells into the Hippocampus
武强 1廖光昊 1李露斯 1范文辉 1黎红华 1濮捷 1徐志鹏 1程鹤 1杨柳 1刘菲1
作者信息
摘要
Abstract
Objective: To observe the migration and differentiation of pEGFP/A2M (FP6) transfected neural stem cells (NSCs), the deposits of Aβ in the brain and the change of learning and memory ability of APP/PS1 double transgenic mice of Alzheimer's Disease (AD) after the NSCs were transplanted into the hippocampus. Methods: APP/PS1 transgenic AD mice were randomly divided into four groups: sham operated (SO) group, artificial cerebrospinal fluid (ACSF) group, transfected pEGFP neural stem cell (pEGFP-NSCs) group and transfected pEGFP/A2M(FP6) neural stem cell (pEGFP/A2M(FP6)-NSCs) group. The ACSF, pEGFP-NSCs or pEGFP/A2M (FP6)-NSCs were transplanted into the CA1 region of the hippocampus of the mice. The learning and memory ability of the mice were assessed with Mirror water maze test. The migration and differentiation of pEGFP/A2M(FP6) transfected NSCs and the deposits of Aβ in the brain of the mice were observed by immuno-histochemistry. Results: The latencies of pEGFP/A2M (FP6)-NSCs group and pEGFP-NSCs group were significantly shorter than that in the SO group and ASCF group (P<0.05). The latency of pEGFP/A2M(FP6)-NSCs group was shorter than that in the pEGFP-NSCs group (P<0.05). Anti-Aβ detection showed Aβ deposits in the hippocampus and cortex of pEGFP/A2M(FP6)-NSCs group and pEGFP-NSCs group were surrounded by transplanted NSCs. The amount and average size of Aβ deposits in the hippocampus and cortex of pEGFP/A2M (FP6)-NSCs group were reduced markedly, compared with the other three groups (P<0.05). The expression of Nestin was detecte after transplantation. Immunofluorescent detection indicated that majority of transplanted cells expressed GFAP while only a few cells expressed MAP-2. Conclusion: Transplantation of pEGFP/A2M (FP6)-NSCs into the hippocampal region of APP/PS1 double transgenic AD mice could reduce the Aβ deposits and promote the learning and memory ability. Partial transplanted NSCs will differentiate into neurons or astro-cytes.关键词
神经干细胞/pEGFP/A2M(FP6)/APP/PS1/双转基因小鼠Key words
neural stem cells/pEGFP/A2M(FP6)/APP/PS1 double transgenic mice分类
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武强,廖光昊,李露斯,范文辉,黎红华,濮捷,徐志鹏,程鹤,杨柳,刘菲..pEGFP/A2M(FP6)转染神经干细胞海马移植治疗APP/PS1双转基因AD小鼠的实验观察[J].神经损伤与功能重建,2013,8(2):103-108,6.
