郑州大学学报（医学版）2013，Vol.48Issue(1)：16-20,5.DOI:10.3969/j.issn.1671-6825.2013.01.004
PTEN基因表达对EC9706细胞增殖、侵袭、凋亡能力的影响
Effects of PTEN on proliferation,invasion and apoptosis in EC9706 cells
摘要
Abstract
Aim :To observe the effects on the proliferation, invasion and apoptosis ability in EC9706 cells when PTEN expression level being changed. Methods : Two PTEN siRNA vectors and one PTEN expression vector were constructed and transfeeted into EC9706 cells respectively. The experimental cells were divided into silent group 1 , silent group 2 , siRNA blank control,PTEN group, PTEN vector control, and untransf'ected group. mRNA and protein expressions of PTEN were measured by RT-PCR and Western blot assay. CCK-8 assay,colony formation assay,flow cytometry and Transwell migration assay were employed to assess the ability of proliferation, colony-forming, apoptosis and migration of EC9706 cells when PTEN expression level was changed. Results: Compared with untransfected group, PTEN mRNA and protein expression levels of silent group 1 and silent group 2 were significantly decreased while that of PTEN group was significantly increased ( F = 25. 762 ,P < 0. 001 ). Compared with untransfected group, the ability of cell proliferation, colony-forming and migration significantly decreased and apoptosis rate increased in PTEN group( F = 50. 752,29. 981 ,32. 193 , P < 0. 001 ). Conclusion : Upregulation of PTEN expression could inhibit EC9706 cells' growth, proliferation, migration and promote apoptosis.关键词
PTEN/EC9706/细胞增殖/细胞侵袭/凋亡/食管癌Key words
PTEN/EC9706/cell proliieration/eell invasion/apoptosis/esophageal carcinoma分类
医药卫生引用本文复制引用
黄丽艳,王娜,汤黎明,许燕,黄幼田,杨红艳,董子明..PTEN基因表达对EC9706细胞增殖、侵袭、凋亡能力的影响[J].郑州大学学报（医学版）,2013,48(1):16-20,5.基金项目
河南省重大公益科研基金资助项目 2011 （）
