郑州大学学报（医学版）2013，Vol.48Issue(1)：28-31,4.DOI:10.3969/j.issn.1671-6825.2013.01.007
慢病毒介导fgl2基因沉默对心肌微血管内皮细胞Ang-1、Ang-2mRNA表达及细胞增殖、迁移的影响
Effects of fibrinogen-like protein 2 gene silencing on expression of Ang-1 and Ang-2 mRNA,proliferation and migration of myocardial microvascular endothelial cell
摘要
Abstract
Airn :To observe the effect of fibrinogen-like protein 2( fgl2 ) gene silencing on myocardial microvascular endothelial cells( MMVECs ) proliferation and cell migration and whether Ang-1 and Ang-2 involve or not. Methods : We constructed a lentiviral RNA interferenced vector of human fgl2 gene. MMVECs were transfected by lentiviral vector. Four days after transfection,quantitative real-time RT-PCR was used to detect the expressions of fgl2,Ang-1 and Ang-2 mRNA,MTT assay was used to detect cell proliferation,and Transwell was used to detect cell migration. Results:The expression of fgl2 mRNA was decreased significantly compared with control group and GFP group( F=180. 301 ,P <0. 001 ). While the expressions of Ang-f and Ang-2 mRNA was increased( F = 59. 450 and 54. 572, P < 0. 001 ). The proliferation and migration ability of MMVECs increased( F =5. 341 and 6. 122, P < 0. 05 ). Conclusion : Fgl2 gene silencing induces MMVECs proliferation and cell migration. The possible mechanism is that fgl2 gene silencing upregulates the expressions of Ang-1 and Ang-2 mRNA.关键词
纤维介素2/短发夹RNA/心肌微血管内皮细胞/血管生成素-1/血管生成素-2Key words
fibrinogen-like protein 2/short hairpin RNA/myocardial microvascular endothelial cell/Ang-1/Ang-2分类
生物科学引用本文复制引用
郑振中,王亮,吴友平,殷然,王梦洪,郑泽琪,彭景添,魏云锋..慢病毒介导fgl2基因沉默对心肌微血管内皮细胞Ang-1、Ang-2mRNA表达及细胞增殖、迁移的影响[J].郑州大学学报（医学版）,2013,48(1):28-31,4.基金项目
国家自然科学基金资助项目 30960119 （）
