郑州大学学报（医学版）2013，Vol.48Issue(1)：47-50,4.DOI:10.3969/j.issn.1671-6825.2013.01.012
伴胃肠动力障碍的重症急性胰腺炎大鼠结肠黏膜下神经节一氧化氮合酶阳性神经元的变化
Change of nitric oxide synthase immunoreactive neurons in colonic submucous ganglia of rats with severe acute pancreatitis complicated with gastrointestinal dysmotility
摘要
Abstract
Airn:To investigate the change of nitric oxide synthase( NOS ) immunoreactive neurons in colonic submu-cous ganglia of rats with severe acute pancreatitis( SAP ) accompanied by gastrointestinal dysmotility. Methods:Twenty SD rats were randomly divided into two groups: sham operation group and model group. SAP model was established by retrograde injecting sodium taurocholate into the bile-pancreatic duct. After 24 h, small intestinal transit index, frequency of colonic peristalisis, water content of the fecal matter, and pathological damage of pancreas were measured. Whole-mount samples were prepared and double immunofluorescence was employed to observe NOS immunoactive neurons in colonic submucous ganglia. Results : Compared with sham operation group, small intestinal transit index, frequency of colonic peristalisis and water content of fecal matter of the model group were significantly lower[ ( 41. 04 ±4. 36 )% vs ( 14. 54 ± 2. 30 )% , ( 13.40 ±1.35 ) min-1 vs ( 4.00 ±0.93 ) min-1 ,( 41. 56 ± 6. 69 )% vs ( 12. 98 ± 6. 56 )% ,t = 15. 477 ,16. 748 ,8. 869, P < 0.001] ,and score of pancreatic pathological damage was higher[ ( 1.20 ±0. 63 ) vs ( 15. 75 ±1,72 ),t =23. 569, P < 0. 001 ], however, there was no signilicant changes in NOS immunoreactive neurons count[ ( 22.49 ±5. 14 )% vs ( 26. 36 ± 2. 57 )% ,Z = - 1. 955 ,P = 0. 055 ]. Conclusion : NOS immunoreactive neurons in colonic submucous ganglia may be no significant plasticity in SAP rats complicated with gastrointestinal dysmotility.关键词
重症急性胰腺炎/黏膜下神经节/一氧化氮合酶/胃肠动力障碍/大鼠Key words
severe aeute pancreatitis/submucous ganglion/nitric oxide synthase/gastrointestinal dysmotility/rat分类
医药卫生引用本文复制引用
袁园,林中,李小玲..伴胃肠动力障碍的重症急性胰腺炎大鼠结肠黏膜下神经节一氧化氮合酶阳性神经元的变化[J].郑州大学学报（医学版）,2013,48(1):47-50,4.基金项目
广西壮族自治区卫生厅中医药科技专项课题 GZKZ10-125 （）
广西壮族自治区科学基金资助项目桂科回0342027 （）
