河南农业科学2013，Vol.42Issue(3)：31-35,5.
高质量花生根尖细胞染色体制片方法研究
Study on Slide Preparation Methods for High Quality Chromosomes from Root Tip Cells of Arachis
摘要
Abstract
In order to get high quality slide preparation to meet the requirements of chromosome banding and in situ hybridization, the cytogenetic slide preparation with evenly dispersing cell layers and well spreading chromosomes was obtained from root tip cells of Arachis. The key procedures included selecting of roots with appropriate size, pretreating with p-dichlorobenzene and fixing with Carnoy's solution, and making slide directly after heating in 45% glacial acid. This method had little effect on chromosome compared with the method of HC1 disintegrating. By saving the costly pectinase and cellulose through omitting steps of enzymatic hydrolysis and hypotonic to cell wall,the method saved time and costs. This method was also applied to chromosome preparation of other crops such as sesame,rice,corn,soybean,green bean and tomato,which again proved to be efficient.关键词
花生/根尖细胞/染色体/醋酸烘烤/制片方法/DAPI染色Key words
Arachis hypogaea / root tip cells/ chromosome/ glacial acid heating/ slide preparation/ DAPI staining分类
农业科技引用本文复制引用
杜培,张新友,李丽娜,黄冰艳,易明林,董文召,汤丰收..高质量花生根尖细胞染色体制片方法研究[J].河南农业科学,2013,42(3):31-35,5.基金项目
河南省重大科技专项(111100110200) （111100110200）
国家科技支撑计划(2011BAD35B04) （2011BAD35B04）
国家花生产业技术体系建设项目(CARS-14) （CARS-14）
