河南农业科学2013，Vol.42Issue(3)：107-111,114,6.
怀地黄液泡质子泵焦磷酸水解酶基因的克隆与生物信息学分析
Cloning and Bioinformatics Analysis of One Vacuolar H+-Pyrophosphatase Gene in Rehmannia glutinosa, f.hueichingensis (Chan et Sehih) Hsiao
摘要
Abstract
In this study,to clone Rehmannia glutinosa functional genes,a pair of degenerate primers was designed using the software CLUSTAL 2. 1 and DNAMAN 4. 0 according to the conserved regions of the five known plant ARGOS base sequences from GenBank nucleic acid database in NCBI. One 495 bp-cDNA fragment of Rehmannia glutinosa. f. hueichingensis (Chan Et Sehih ) Hsiao was cloned using RT-PCR. BLAST analysis indicated that its base sequence was homologous to those of seven known plant vacuolar H+-pyrophosphatase (V-PPase) genes from GenBank with an identity of 83%-86% ,showing that it was V-PPase gene in Rehmannia glutinosa. f. hueichingensis(Chan Et Sehih) Hsiao.关键词
怀地黄/液泡质子泵焦磷酸水解酶/基因克隆/cDNA末端快速扩增技术/生物信息学分析Key words
Rehmannia glutinosa f. hueichingensis (Chan et Sehih) Hsiao/ vacuolar H+-pyro-phosphatase(V-PPase) / gene cloning/ RACE/ bioinformatics analysis分类
农业科技引用本文复制引用
周延清,张永华,陈艳梅,张喻,李静云,陈娟娟,白妍妍,魏海方..怀地黄液泡质子泵焦磷酸水解酶基因的克隆与生物信息学分析[J].河南农业科学,2013,42(3):107-111,114,6.基金项目
河南省基础与前沿技术研究计划项目(092300410009) （092300410009）
河南省教育厅自然科学研究计划项目(科技攻关)(2011A180022) （科技攻关）
河南师范大学第三届大学生创新创业论坛暨大学生创新性实验计划项目 （）
