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AB-8大孔树脂纯化鸡骨草总皂苷的工艺研究

廖春燕 黄瑶 杨晓

河南农业科学2013,Vol.42Issue(3):150-153,4.
河南农业科学2013,Vol.42Issue(3):150-153,4.

AB-8大孔树脂纯化鸡骨草总皂苷的工艺研究

Purification Process of Total Saponins in Abrus cantoniensis Using AB-8 Macroporous Resin

廖春燕 1黄瑶 1杨晓1

作者信息

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摘要

Abstract

In order to improve the purification efficiency of total saponins in Abrus cantoniensis, the conditions and parameters of purification process of total saponins using AB-8 macroporous adsorption resin were studied. The conditions of absorption and desorption were investigated with the indexes;absorption ratio and reserved rate of total saponins. The results showed that the sample loading solution was 1. 00 g/L of 2. 0 BV with pH value of 6. 0 at the sample loading flow rate of 2. 0 BV/h. The elution solvent was 3. 5 BV of 50% ethanol solution with pH value of 7. 0 at flow rate of 4. 0 BV/h. The total saponins reserved rate was 82. 8%, with the purity of 75.18%.

关键词

鸡骨草/总皂苷/AB-8大孔树脂/纯化

Key words

Abrus cantoniensis/ total saponins/ AB-8 macroprous resin/ purification

分类

农业科技

引用本文复制引用

廖春燕,黄瑶,杨晓..AB-8大孔树脂纯化鸡骨草总皂苷的工艺研究[J].河南农业科学,2013,42(3):150-153,4.

基金项目

广西科技大学(筹)科学基金资助项目(校科自1261103) (筹)

河南农业科学

OA北大核心CSCDCSTPCD

1004-3268

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