核农学报2013，Vol.27Issue(2)：152-157,6.
花生种子芽期耐低温相关基因克隆
Cloning and Analysis of Differentially Expressed Genes from Peanut in Response to Chilling Stress
摘要
Abstract
Differences in gene expression between seeds of Huayu 44, a large type peanut line with chilling tolerance imbibed in 2℃ water and seeds of the same cultivar treated with 15℃ water (control), were studied using the GeneFishing technology. Two differentially expressed genes ACP20 and ACP2 were isolated. BLAST showed that they had high homology to the genes encoding oleosin and nifU-like protein, respectively. Expressions of both genes were studied by real time RT-PCR, and complete coding sequences of both genes were obtained by rapid amplification of cDNA ends. Preliminary biological functions of the two genes were further predicted. The study can help us to understand the molecular mechanism underlying resistance and facilitate breeding process.关键词
花生/耐低温/相关基因/差异表达Key words
Peanut/ Chilling tolerance/ Related gene/ Differential expression引用本文复制引用
王秀贞,唐月异,吴琪,高华援,胡东青,张树伟,陈殿绪,戚永春,王传堂..花生种子芽期耐低温相关基因克隆[J].核农学报,2013,27(2):152-157,6.基金项目
现代农业产业技术体系建设专项资金(CARS-14),青岛市科技计划项目(12-1-4-11-(1)-jch,12-1-4-11 (3)-jch),吉林省自然科学金基金(201215195) （CARS-14）
