核农学报2013，Vol.27Issue(2)：231-239,9.
干旱胁迫及复水对棉花叶片氮代谢的影响
Effect of Progressive Drought Stress and the Subsequent Re-watering on Leaf Nitrogen Metabolism Cotton Seedlings
摘要
Abstract
Using two genotypes cotton; Xinluzao 7 and Xinluzao 24(drought - resistance and non drought - resistance respectively)materials, some key enzymes activity involving nitrogen metabolism, soluble proteins, total nitrogen, ammonium and free amino acid contents were investigated under progressive drought and re-watering treatment. Progressive drought resulted in NR activity decreased from 51. 67% to 74. 22% , GS activity from 50. 65% to 72. 95% , GOGAT activity from 43. 11% to 68. 36% in Xinluzao 7 and Xinluzao 24 leaves, compared with control, respectively. GDH activity reaches a maximum on the fourth day. Whereas endopeptidase activity increased from 115. 76% to 165. 06% , total nitrogen content from 11. 39% to 8. 43% , soluble proteins from56. 96% to 34. 12% , ammonium content from 92. 29% to 111. 56% in Xinluzao 7 and Xinluzao 24 leaves, compared with control, respectively. Furthermore,the free amino acids content increased slowly during the early stage of drought treatment and then increased rapidly during the latter stage of drought treatment in Xinluzao 7, while the situation was opposite in Xinluzao 24. After re-watering treatment, four indexes recovered more rapidly, including NR activity, GS activity, GOGAT activity and GDH; but the other four indexes decreased more rapidly, including endopeptidase activity, free amino acids content, soluble proteins and total nitrogen content in Xinluzao 7 than Xinluzao 24. These results suggested that cotton with high drought resistance may has stronger ammonium assimilative capacity, thus it could increased osmotic adjusted substance ( especially proline) to improve drought resistance.关键词
干旱胁迫/复水/棉花叶片/氮代谢Key words
Drought stress/ Re-watering/ Cotton leaves/ Nitrogen metabolism引用本文复制引用
曹让,梁宗锁,吴洁云,张旺锋,许加..干旱胁迫及复水对棉花叶片氮代谢的影响[J].核农学报,2013,27(2):231-239,9.基金项目
国家自然科学基金(31000675,U1203283) （31000675,U1203283）
