家畜生态学报2013,Vol.34Issue(2):38-40,3.
用异源蛋白和蛋壳体外培养鹌鹑胚胎的研究
Study on the Quail Embryo Culture In Vitro by Foreign Albumen and Eggshell
摘要
Abstract
Fifty quail embryos with egg yolk but without albumen were used to examine the possibility of its culture by thin albumen from chicken eggs in the chicken eggshell. The quail embryos were cultured in an incubator at 38. 0℃ and 60% for RH from day 1 to 2. 5, 37. 8℃ and 55% for RH from days 2. 5 to 14, 37. 5℃ and 70% for RH from days 15 to 17. The chicken eggshells with quail embryos were rocked at an angle of 90 for the first 2. 5 days and 50 from day 2. 5 to 14, once every hour. Stopped turning after 14 days. The survival rate of the quail embryos was 92% at day 2. 5, 78% at day 15. The hatchability was 28%. The result suggested that thin albumen from chicken eggs was not rejected by the quail embryo, and it was a good in vitro culture medium for the quail embryo.关键词
鹌鹑/胚胎/培养/鸡稀蛋白/鸡蛋壳Key words
quail/embryo/culture/thin albumen from chicken eggs/chicken eggshell分类
农业科技引用本文复制引用
张文昌,李玉芳,庄益芬..用异源蛋白和蛋壳体外培养鹌鹑胚胎的研究[J].家畜生态学报,2013,34(2):38-40,3.基金项目
福建省自然科学基金项目(2007J0042) (2007J0042)