基础医学与临床2013，Vol.33Issue(3)：303-307,5.
转染NY-ESO-1特异性TCR增强人PBMC对NY-ESO-1阳性肿瘤细胞的特异性细胞毒性
Transfection of NY-ESO-1 specific TCR improves the specific cytotoxicity of human PBMC to NY-ESO-1 positive tumor cells
摘要
Abstract
Objective To study whether NY-ESO-1-specific TCR gene transduced into human PBMC (Peripheral Blood Mononuclear Cell, PBMC) can improve its abilities of specifically recognizing and killing tumor cells. Methods pCDNA3. 1-ESO-TCR plasmid was electroporately transferred into PBMC separated from healthy people in vitro and confirmed by RT-PCR. The phenotype analysis after eletroporation was measured by flow cytometry method. The NY-ESO-1 specific peptide (pl57-165) was added into the culture of electroporated PBMC, the IFN-γ level secreted by electroporated PBMC was detected by ELISPOT assay. The specific cytotoxicity in vitro was detected by real time cell analysis (ACEA biosciences). Results The NY-ESO-1-specific TCR fragments in electroporated PBMC were detected by RT-PCR. The specific expression of NY-ESO-TCR in transferred PBMC was significantly higher than that in PBMC transfered with empty vector (P < 0. 05). The positive dots of IFN-γ secretion in transferred PBMC stimulated by peptide pl57-165 was significantly more than that in PBMC transfered with empty vector (P <0. 05); The specific cytotoxicity of transferred PBMC was obviously enhanced than PBMC transduced with empty vector. Conclusions The specific cytotoxicity of PBMC against NY-ESO-1 positive HepG2 cells is elevated by transduced PBMC with NY-ESO-1 specific TCR, which may stimulate more researches about adoptive immunotherapy of hepatocellular carcinoma.关键词
TCR/PBMC/电转/NY-ESO-1Key words
TCR/ PBMC/ electroporation/ NY-ESO-1分类
医药卫生引用本文复制引用
郭佳,田洲,顾娜,徐珩,闾军..转染NY-ESO-1特异性TCR增强人PBMC对NY-ESO-1阳性肿瘤细胞的特异性细胞毒性[J].基础医学与临床,2013,33(3):303-307,5.基金项目
北京市自然科学基金(7092044) （7092044）
首都医学发展科研基金(2009-3156) （2009-3156）
北京市卫生系统高层次卫生技术人才培养项目资助(2011-J.L) （2011-J.L）
