基础医学与临床2013，Vol.33Issue(3)：308-313,6.
cPKCγ在HPC抗N2a细胞OGD损伤中作用及其分子机制
cPKCγ in HPC protects N2a cells against OGD-induced injury and its molecular mechanism
摘要
Abstract
Objective To explore the role of conventional protein kinase C (cPKC) γ in hypoxic preconditioning (HPC) protecting mouse N2A neuroblastoma cells against oxygen-glucose deprivation (OGD)-induced ischemic injuries in vitro and potential molecular mechanism. Methods By establishing hypoxic preconditioning (HPC) and oxygen-glucose deprivation (OGD) N2a cell models, and using thiazolyl blue tetrazolium bromide (MTT), lactate dehydrogenase (LDH) assay, terminal deoxynucleotidyl transferase (TdT) -mediated dUTP nick end labeling (TUNEL) staining and Western blot for LC3 Ⅱ/Ⅰ to detect the level of N2a cell injury, necrosis, apoptosis and autophagy, respectively. Results OGD 2 and 4 h significantly affected the viability and mortality of N2a cells, respectively (P <0. 05, n =6 per group); HPC (20 min) may protect the N2a cells against OGD 3 h-induced ischemic injuries, but the cPKCγ inhibitor Go6983b (6 nmol/L) abolished this neuroprotection of HPC (P < 0. 05, n = 16); OGD 3 h significantly increased the apoptosis numbers (P < 0. 05, n = 6), but neither HPC nor HPC + Go6983 affect OGD 3 h-induced cell apoptosis of N2a cells; OGD 3 h enhanced the autophagy level of N2a cells, Go6983 and HPC + Go6983, but not HPC alone, inhibited OGD 3 h-induced cell autophagy (P < 0. 05, n =6); HPC could significantly inhibit OGD 3 h-induced cell necrosis, but cPKCγ inhibitor Go6983 (HPC + Go6983) abolished this HPC-induced neuroprotection (P <0. 05, n = 16). Conclusions These results demonstrated that cPKCγ plays an important role in HPC protecting N2a cells against OGD-induced ischemic injuries, and this neuroprotective effect of HPC is mainly attributed to the reduction in necrosis of OGD treated N2a cells.关键词
低氧预适应(HPC)/氧糖剥夺(OGD)/细胞凋亡/细胞自噬/细胞坏死Key words
hypoxic preconditioning (HPC)/ oxygen-glucose deprivation (OGD)/ cell apoptosis/ cell autophagy/ cell necrosis分类
医药卫生引用本文复制引用
罗宏,苏吉儿,韩松,李俊发..cPKCγ在HPC抗N2a细胞OGD损伤中作用及其分子机制[J].基础医学与临床,2013,33(3):308-313,6.基金项目
国家自然科学基金(31071048,31171147) （31071048,31171147）
国家重点基础性研究项目(2012CB18200) （2012CB18200）
科技部973计划前期研究专项(2011CB512109) （2011CB512109）
