基础医学与临床2013，Vol.33Issue(3)：341-345,5.
螺内脂降低大鼠心肌非梗死区MMP/TIMP基因转录及表达
Spirolactone decreases transcription and expression of MMPs/TIMP-2 gene in myocardial noninfarct area of rat
摘要
Abstract
Objective To investigate the effects of the aldosterone receptor antagonism spironolactone on the relationship between MMPs/TIMPs expression and activity remodeling after myocardial infraction (MI) and to explore the molecular mechanism of heart failure prevention after MI. Methods Myocardial infarction model was set up by drug intervention. Histopathology was detected by HE staining. Immunohistochemical and RT-PCR were used to observe the gene and protein expression of MMP-2, MMP-9, TIMP-2. Results Post infarction both compare MI and control MMP-2, MMP-9, TIMP-2 was significantly increased in 48 h and then remains at higher level for 4 weeks (P <0. 01). MMP-2 and MMP-9 patency fallout, TIMP-2 no-apparent fallout by spirolactone interference. Conclusions Spirolactone can decrease transcription and expression of MMP-2, MMP-9/TIMP-2 activity in myocardial noninfarct area of rat.关键词
心肌梗死/螺内酯/基质金属蛋白酶/基质金属蛋白酶抑制剂/心室重构Key words
myocardial infarction/ spirolactone/ matrix metalloproteinases/ tissue inhibitors of metalloproteinases/ ventricular remodeling分类
医药卫生引用本文复制引用
王全志,杨旸,陈惠军,杨廷桐..螺内脂降低大鼠心肌非梗死区MMP/TIMP基因转录及表达[J].基础医学与临床,2013,33(3):341-345,5.基金项目
河南省科技厅重点攻关项目(082102310082) （082102310082）
