吉林农业大学学报2013，Vol.35Issue(2)：171-176,6.
侧耳RSAP-PCR体系的建立和优化
Establishment and Optimization of RSAP-PCR Reaction Conditions in Pleurotus ostreatus
摘要
Abstract
In this study, single factor and orthogonal experiments were used to establish and optimize the RSAP - PCR reaction system of Pleurotus ostreatus. Then, experiment tests were done to check the stability of the RSAP - PCR reaction system using a different pair of primers. Finally, a 25 μL optimized reaction system was obtained: 30 ng DNA, 2.0 μL 10 × PCR buffer, Mg2+ 2.5 mmol/L, dNTPs 0.25 mmol/L, Primers 0.32 μmol/L, Taq DNA polymerase 2 U, ddH2O 13.2 μL.关键词
侧耳/RSAP/体系优化Key words
Pleurotus ostreatus/RSAP/system optimization分类
农业科技引用本文复制引用
潘祖桐,宋慧,刘麒,李胜南,李媛媛,刘晓龙..侧耳RSAP-PCR体系的建立和优化[J].吉林农业大学学报,2013,35(2):171-176,6.基金项目
吉林省农委项目(10096) （10096）
