吉林农业大学学报2013，Vol.35Issue(2)：184-187,4.
重组NDV HN基因乳酸菌穿梭表达载体的构建及在大肠杆菌中的表达
Construction of Recombinant Newcastle Disease Virus HN Lactobacillus Shuttle Expression Vector and Expression in Escherichia coli
摘要
Abstract
To express a recombinant Newcastle disease virus Lactobacillus expression vector, HN gene was amplified by RT - PCR from DNV and cloned into pMD18-T Simple Vector. After receiving the right sequence, the particle was further recombined with Lactobacillus expression vector and then transformed into E. coli BL21 (DE3) . The result showed that NDV HN gene was correctly cloned into Lactobacillus expression vector and the recombinant strain could be obtained for at least 50 generations. SDS - PAGE and Western blotting results displayed that NDV HN had reactionogenicity with NDV positive antibody, indicating that recombinant NDV HN Lactobacillus expression vector was constructed.关键词
新城疫病毒/血凝素-神经氨酸酶/乳酸菌穿梭表达载体/大肠杆菌Key words
NDV/hemagglutinin-neuraminidase/Lactobacillus expression vector/Escherichia coli分类
农业科技引用本文复制引用
郭衍冰,胡静涛,于丹,曹岩峰,王一鸣,张旺,王春凤..重组NDV HN基因乳酸菌穿梭表达载体的构建及在大肠杆菌中的表达[J].吉林农业大学学报,2013,35(2):184-187,4.基金项目
吉林省科技发展计划项目(201101112),吉林省教育厅"十二五"科学技术研究项目(201149),国家高技术研究发展计划项目(2011AA10A209),教育部博士点基金项目(20110061110005),人兽共患病教育部重点实验室开放平台基金项目,吉林农业大学大学生科技创新基金项目 （201101112）
