吉林农业大学学报2013，Vol.35Issue(2)：188-191,4.
重金属镉离子人工抗原的合成与鉴定
Synthesis and Identification of Artificial Antigen for Heavy Metal Cadmium Ions
摘要
Abstract
Cadmium ions were coupled to Keyhole Limpet Hemocyanin(KLH) and Bovine Serum Albumin (BSA) via Isothiocyanobenzy-EDTA to gain complete antigen (Cd-ITCBE-KLH and Cd-ITCBE-BSA). The antigen concentration was measured by BCA method; ultraviolet scan of spectrophotometer and SDS-PAGE electrophoresis qualitative identification were done and the content of cadmium ions in antigen was determined by GFAAS method. The actual protein concentration of Cd-ITCBE-BSA, Cd-ITCBE-KLH and ITCBE-BSA were in the sequence of 1.426,1.504 and 0.890 mg/mL, ultraviolet scan and electrophoresis identification proved that the antigen synthesis was successful. Concentration of cadmium ion in complete antigen was measured as high as 44.56 μg/mL, and that of cadmium ion in ITCBE-BSA, BSA, KLH and Hepes were almost zero, which indicated the hapten was surely coupled into protein, and further testified the antigen synthesis was successful.关键词
重金属/镉/人工抗原Key words
heavy metal/cadmium/artificial antigen分类
资源环境引用本文复制引用
韩盈盈,李小兵,刘国文,孔涛,郎广平,王哲..重金属镉离子人工抗原的合成与鉴定[J].吉林农业大学学报,2013,35(2):188-191,4.基金项目
吉林省世行贷款农产品质量安全项目(2011-Z42) （2011-Z42）
