吉林农业大学学报2013，Vol.35Issue(2)：198-205,8.
山葡萄SRAP技术体系的建立及其在品种鉴定中的应用
Establishment of SRAP-PCR System and Variety Identification on Vitis amurensis
摘要
Abstract
With "zuoyouhong" and five different cultivars of grape as materials, the optimization of SRAP molecular marker system of Vitis amurensis was carried out, and the feasibility of SRAP marker in identifying grape cultivars was discussed. The main results are as follows: SRAP amplification program: 94 ℃ denaturation for 5 min; the first 5 cycles, 94 ℃ denaturation 45 s, 35 ℃ annealing 45 s, 72℃ extension 90 s; after 35 cycles, 94 ℃ denaturation 45 s, 50℃ annealing 45 s, 72 ℃ for 90 s; last 72 ℃ for 7 min. Reaction system: 25 μL total reaction system containing template DNA 20 ng, dNTPs 1.0 μL, upstream and downstream primers each 1 μL, Taq DNA polymerase 0,2 μL, 10 × Buffer 1.0 μL, the other with ddH2O added. Ninety-six bands were produced from six different cultivars of Vitis amurensis by 16 pairs of selected primers, and the percentage of polymorphic bands (PPB) was 67.71%. Each species has its unique band, at least with 2 pairs of primers Vitis amurensis cultivars can be identified.关键词
山葡萄/SRAP/体系建立/品种鉴定Key words
Vitis amurensis/SRAP/system establishment/cultivar identification分类
农业科技引用本文复制引用
金炳奎,宗成文,曹后男,朴日子,许雪..山葡萄SRAP技术体系的建立及其在品种鉴定中的应用[J].吉林农业大学学报,2013,35(2):198-205,8.基金项目
延边大学科技发展计划项目(2008-11) （2008-11）
