解剖学杂志2013，Vol.36Issue(2)：152-155,4.DOI:10.3969/j.issn.1001-1633.2013.02.005
胰岛素样生长因子-1对白介素-1体外诱导家兔椎间盘髓核细胞凋亡的影响
Effect of insulin-like growth factor-1 on the apoptosis of rabbit nucleus pulposus cells induced by interleukin-1
摘要
Abstract
Objective:To investigate the effects of insulin-like growth factor-1 (IGF-1) on the apoptosis of rabhits nucleus pulposus cells induced by interleukin-1 (IL-1). Methods:The nucleus pulposus was removed from intervertebral discs of healthy domestic rabbits. The cells isolated from these structures were cultured to a monolayer, and then digested with collagenase II and purified using trypsin and differential adhesion methods. Nucleus pulposus cells were then identified using type II collagen immunohistochemistry and were stained with toluidine blue. Following exposure to IGF-1 or IL-1, they were observed by light microscopy. Giemsa staining, TdT-mediated dUTP-biotin nick end labeling (TUNEL), and flow cytometry were used to detect the rate of early cell death, which served as an indicator of apoptosis. Results:A large number of nucleus pulposus cells underwent apoptosis. Morphological changes typically associated with apoptosis were observed in the IL-1 group without obvious signs of apoptosis. A small but significant degree of apoptosis was seen in the IGF-1 group. TUNEL revealed that the rate of apoptosis in the IGF-1 group was significantly reduced compared with the IL-1 group, which was confirmed using flow cytometry. Compared with the control group, the rate of apoptosis was also significantly increased in IL-1-exposed cells. Conclusion:These findings suggest that IGF-1 inhibits the apoptosis of nucleus pulposus cells induced by IL-1.关键词
椎间盘退变/髓核/凋亡/胰岛素样生长因子-1/白介素-1/原位末端标记Key words
intervertebral disc degeneration/nucleus pulposus/apoptosis/insulin- like growth factor-1/interleukin-1/TUNEL引用本文复制引用
张长春,周建生,王祥,周新社,孙宝安,邵晨,林清华..胰岛素样生长因子-1对白介素-1体外诱导家兔椎间盘髓核细胞凋亡的影响[J].解剖学杂志,2013,36(2):152-155,4.基金项目
安徽省高校省级自然科学研究重点项目(KJ2011A204) （KJ2011A204）
