解剖学杂志2013，Vol.36Issue(2)：163-165,3.DOI:10.3969/j.issn.1001-1633.2013.02.008
大鼠肝癌发生过程中肿瘤坏死因子相关的凋亡诱导配体及核转录因子-κB的表达
Expression of tumor necrosis factor-related apoptosis inducing ligand and nuclear factor-κB during hepatocarcinogenesis in rats
摘要
Abstract
Objective:To investigate the expression and role of tumor necrosis factor-related apoptosis inducing ligand (TRAIL) and nuclear factor-κB (NF-κB) during hepatocarcinogenesis in rats. Methods:The dynamic expression of TRAIL and NF-κB during hepatocarcinogenesis induced by diethylnitrosamine (DEN) in rats was measured by using immunohisto-chemical SP method. Results:Hepatocellular carcinoma was induced by DEN. The procession of hepatocarcinogenesis in this model included three-stage hepatic toxin lesion, hepatic proliferation/cirrhosis and hepatic carcinogenesis. Only a few TRAIL positive cells were shown ocassionally in the normal liver tissues, toxin lesion and hepatic proliferation/cirrhosis stages. On the hepatic carcinogenesis stage, more TRAIL positive cells were seen mainly located within cancer nodules. An increasing tendency of NF-κB expression was shown from the normal liver to precancerous to cancerous tissues with a progress of rat hepatocarcinogenesis and a large number of NF-κB positive cells was shown at the terminal cancer stage compared to the lower expression in the normal liver tissues. Conclusion:The results suggest that TRAIL and NF-κB in hepatocytes are closely related to hepatocarcinogenesis.关键词
肿瘤坏死因子相关的凋亡诱导配体/核转录因子-κB/肝癌发生/免疫组织化学/大鼠Key words
tumor necrosis factor-related apoptosis inducing ligand/nuclear factor-κB/hepatocarcinogenesis/immunohisto-chemistry/rat引用本文复制引用
李京敏,宁巍巍,李笑岩,刘同慎,白咸勇..大鼠肝癌发生过程中肿瘤坏死因子相关的凋亡诱导配体及核转录因子-κB的表达[J].解剖学杂志,2013,36(2):163-165,3.基金项目
国家自然科学基金(81073123) （81073123）
山东省自然科学基金(ZR2009CL003,Q2008C01) （ZR2009CL003,Q2008C01）
