解剖学杂志2013，Vol.36Issue(2)：177-180,4.DOI:10.3969/j.issn.1001-1633.2013.02.012
鸡胚视顶发育过程中神经元增殖、迁移和分层形成的规律
Characterization of proliferation, migration and layer formation of neurons in chicken optic tectum during development
杨慈清 1杜蕊 1张学乾 1林俊堂 1赵善廷1
作者信息
摘要
Abstract
Objective:To characterize the proliferation, migration and layer formation of neurons in chicken optic tectum by BrdU-labeling. Methods:The fertilized eggs were incubated in ovo for three days and one window was open upside. BrdU (1 mg/ml) was dropped from 4 to 18 incubation days, and five eggs were selected from each group when incubated at day 18. All samples were collected and fixed with 4% paraformaldehyde and sliced with vibratome. Newborn neurons were stained by thionin and immunohistochemistry to show BrdU labeled neurons. To comprehensively compare the differences in various stages of newborn neurons, six different visual areas were selected by bio-statistics software to analyze the number of proliferating cells, the total area, the average gray level, the average optical density and the average integral optical density in stratum griseum et fibrosum superficiale layer. Results:The chick embryo optic tectum structure was clearly divided into six layers. The stage of cell proliferation was at 4 d-8 d during embryonic development, the number of new cells was significantly higher than that in other stages, and newborn cells was significantly reduced after 12 d. Conclusion:During the chick embryo development, the development of optic tectum begins at 4 d and ends at 12 d, and the structure of 6 layers is formed after 12 d. The newborn neurons are decreased significantly after 12d and the the six-layer structure formation is arranged from the inside to outside.关键词
鸡胚/5溴脱氧尿嘧啶核苷/视顶盖/增殖/神经元迁移Key words
chick embryo/5-bromodeoxyuridine/optic tectum/proliferation/neuronal migration引用本文复制引用
杨慈清,杜蕊,张学乾,林俊堂,赵善廷..鸡胚视顶发育过程中神经元增殖、迁移和分层形成的规律[J].解剖学杂志,2013,36(2):177-180,4.
