解剖学杂志2013，Vol.36Issue(2)：181-184,223,5.DOI:10.3969/j.issn.1001-1633.2013.02.013
神经病理性疼痛中星形胶质细胞被激活后的p38丝裂原活化蛋白激酶信号转导通路
p38MAPK signaling pathways after activation of the astrocytes in neuropathic pain
摘要
Abstract
Objective:To investigate p38 mitogen-activated protein kinase (p38MAPK) signaling pathways after astrocytes were activated in neuropathic pain. Methods:20 SD rats were randomly divided into two groups:a model group of chronic constriction injury (CCI) in the sciatic nerve and a sham operation group. In every group, No. 4-5 lumbar spinal cord was taken to make paraffin slices at 1 day before operation and 1, 3, 7, 14 days after operation. The expression of p38MAPK was labeled by immunofluorescence method, and then the relationship between the nerve cells of the spinal cord and p38MAPK was detected with double immunolabeling technique. Results:The p38MAPK positive cells were increased in the number in the ipsilateral spinal dorsal horn of the operative limb in the CCI group. The mean fluorescence intensity of the p38MAPK was significantly increased compared with others, which reached the highest at 7 days after operation. The p38MAPK and micro-glia had a good consistence in the spinal dorsal horn in the CCI group. Conclusion:In the neuropathic pain, p38MAPK signaling pathways were activated, but the p38MAPK signal transduction pathways were not involved in pain signaling transduction of astrocytes.关键词
p38丝裂原活化蛋白激酶/神经病理性疼痛/星形胶质细胞/小胶质细胞Key words
p38 mitogen-activated protein kinase/neuropathic pain/astrocyte/microglia引用本文复制引用
王江栓,曹靖,李变锋,任秀花,刘晓东,臧卫东..神经病理性疼痛中星形胶质细胞被激活后的p38丝裂原活化蛋白激酶信号转导通路[J].解剖学杂志,2013,36(2):181-184,223,5.基金项目
河南省基础与前沿项目(102300410004) （102300410004）
河南省教育厅自然科学研究项目(2010A310009) （2010A310009）
