解剖学杂志2013，Vol.36Issue(2)：192-196,5.DOI:10.3969/j.issn.1001-1633.2013.02.016
经鼻及皮下给予睾丸酮/丙酸睾丸酮对大鼠相关脑区c-Fos表达效能的影响
Effects of nasal administration or subcutaneous injection of testosterone/testosterone propionate on expression efficacy of c-Fos in rat brain
摘要
Abstract
Objective:To explore the efficacy of intranasal administration or subcutaneous injection of testosterone (T) / testosterone propionate (TP) on different brain regions in rats. Methods:Radioimmunoassay was used to detect testosterone concentration in cerebrospinal fluid and in serum after intranasal administration of TP. Immunohistochemistry was used to detect the expression of c-Fos protein in different brain regions. Results:Testosterone in cerebrospinal fluid and in serum was decreased in gonadectomized (GDX) rats compared to the normal control rats. Subcutaneous injection of TP in GDX rats only increased testosterone in serum. Testosterone in cerebrospinal fluid and in serum was increased after intranasal administration of TP in GDX rats and in the normal control rats. Testosterone in cerebrospinal fluid of GDX rats after intranasal administration of TP was higher than that in GDX rats after subcutaneous injection of TP, however testosterone in serum of GDX rats after intranasal administration of TP was lower than that in GDX rats after subcutaneous injection of TP. After intranasal administration of TP or T, the number of c-Fos-immunoreactive cells and the c-Fos immunoreactive intensity were increased in more brain regions. However, after subcutaneous injection of TP, the number of c-Fos-immunoreactive cells and the c-Fos immunoreactive intensity were increased only in a few brain regions. Conclusion:Intranasal administration of TP or T could induce the expression of c-Fos and activate more brain regions. That can provide a new therapy method for some central nervous system diseases.关键词
睾丸酮/丙酸睾丸酮/鼻腔给药/脑脊液/血清/c-FosKey words
testosterone/testosterone propionate/intranasal administration/cerebrospinal fluid/serum/c-Fos引用本文复制引用
张国梁,牛小龙,康云霄,薛岩,方卉,石葛明..经鼻及皮下给予睾丸酮/丙酸睾丸酮对大鼠相关脑区c-Fos表达效能的影响[J].解剖学杂志,2013,36(2):192-196,5.基金项目
河北省自然科学基金(C2012206033) （C2012206033）
