昆虫学报2013，Vol.56Issue(2)：186-194,9.
利用mtCOI PCR-RFLP技术鉴定中国境内九个烟粉虱隐种
Identification of nine cryptic species of Bemisia tabaci (Hemiptera:Aleyrodidae) from China by using the mtCOI PCR-RFLP technique
摘要
Abstract
Bemisia tabaci (Gennadius) is a species complex containing over 31 cryptic species, some of which are worldwide invasive insect pests. At present, two invasive cryptic species and 13 indigenous cryptic species have been recorded from China. Rapid and efficient methods for identifying cryptic species are important for investigating the population abundance of various cryptic species in the field and development of management strategies. However, apart from the method of sequencing mtCOI and gene sequence BLAST search, an efficient method for indentifying multiple species is still lacking at present. In this study, we adopted the PCR-RFLP technique, using one or more of the five restriction enzymes, namely Taql, Vspl, Van9ll, Ncol and Fokl, to identify nine whitefly cryptic species from China. The results showed that two cryptic species ME AMI and China 1 could be identified through digestion with only Taql, two cryptic species MED and Asia 1 could be identified through sequential digestion with Taql + Ncol, two cryptic species Asia II 3 and Asia II 9 could be identified through sequential digestion with Taql + Van911, and three cryptic species Asia II 1, Asia II 6 and Asia II 7 could be identified through sequential digestion with Taql + Vspl + Fokl. This study provides an efficient method for identifying nine cryptic species of B. tabaci from China.关键词
烟粉虱/隐种/分子鉴定/限制性内切酶/mtCOI/PCR-RFLPKey words
Bemisia tabaci/ cryptic species/ molecular identification/ restriction enzyme/ mtCOI/ PCR-RFLP分类
生物科学引用本文复制引用
秦丽,王佳,邴孝利,刘树生..利用mtCOI PCR-RFLP技术鉴定中国境内九个烟粉虱隐种[J].昆虫学报,2013,56(2):186-194,9.基金项目
国家"973"计划项目(2009CB119203) （2009CB119203）
国家自然科学基金项目(31272104) （31272104）
