昆明医科大学学报2013，Vol.34Issue(1)：23-27,5.
SDF-1/CXCR4在恶性胶质瘤细胞体外增殖、迁移及侵袭中的作用
The Role of SDF-1/CXCR4 Axis in Proliferation, Migration and Invasion of Malignant Glioma Cell Lines
摘要
Abstract
Objective To investigate the role of chemokine SDF-1 and its receptor CXCR4 in malignant glioma cell proliferation, invasion and migration, so as to provide basis for clinical prevention and treatment. Methods Immunohistochemistry was used to detect the CXCR4 expression in malignant glioma cells. MTT assay was used to test different concentration of SDF-1 promoted in vitro proliferation of C6 cells malignant glioma. CXCR4 receptor inhibitor AMD3100 and SDF-1 were co-cultured to test cell proliferation and CXCR4 resistance in malignant glioma by observing the above indexes. The cell migration and invasion ability of C6 in different groups was tested by transwell migration and invasion assay. Results CXCR4 expressed positively in malignant glioma cell line C6. SDF-1 promoted C6 cells proliferation, invasion and migration in vitro. Anti-CXCR4 McAb could inhibit the proliferation, invasion and migration in a dose-dependent manner. AMD3100 inhibited the effects induced by SDF-1. Conclusion The role of SDF-1/CXCR4 in malignant glioma cell lines proliferation, invasion and migration in vitro is related to invasion and migration of malignant glioma, and may be a new therapeutic target for glioma.关键词
恶性胶质瘤C6细胞系/趋化因子SDF-1/增殖/迁移Key words
Malignant glioma cell line C6/ SDF-1/ Proliferation/ Migration分类
医药卫生引用本文复制引用
刘佳鑫,王崇谦,王伟民,丁鹏,王波,牟临杰,王进昆..SDF-1/CXCR4在恶性胶质瘤细胞体外增殖、迁移及侵袭中的作用[J].昆明医科大学学报,2013,34(1):23-27,5.基金项目
云南省科技计划联合专项基金资助项目(2010CD157) （2010CD157）
