兰州大学学报（医学版）2012，Vol.38Issue(3)：17-22,26,7.
RNA干扰沉默编码跨膜蛋白35基因表达对肝癌细胞生长及细胞周期的影响
Effect of coding transmembrane protein 35 gene on cell proliferation and cell cycle in hepa-toma cells by RNA interference
摘要
Abstract
Objective To study the role of transmembrane protein 35 (TMEM35) gene in cell growth, cell cycle of hepatoma cells, and to discuss the feasibility of TMEM35 gene encoding a membrane protein as a potential therapy target of hepatoma. Methods Real-time quantitative polymerase chain reaction was performed to examine TMEM35 expression in tumor tissues and non-tumor ones of patients with liver cancer. siRNAs were chemically synthesized to interfere TMEM35 in MHCC-97H and MHCC-97L cells. Soft-agar colony formation as-say was performed to evaluate cell growth, and cell cycle was analyzed by flow cytometry. TMEM35 protein was analyzed in functional interaction networks database to predict functional interaction genes of TMEM35. Results TMEM35, as a tetraspanin, expressed abnormally in liver cancers. From 34 examined patients, TMEM35 expression increased in 14 and decreased in 20 significantly (P <0.05). Two siRNAs against TMEM35 gene were designed and synthesized to specially knockdown TMEM35 in MHCC-97H and MHCC-97L cells. TMEM35 knockdown inhibited cellular soft-agar colony formation in MHCC-97H and MHCC-97L cells. And flow cytometry analysis revealed that TMEM35 knockdown by RNA interference could result in G1 arrest and S decrease. Conclusion TMEM35 gene, which encodes a tetraspanin, plays an important role in cell growth of hepatoma cells.关键词
RNA干扰/四跨膜蛋白/肝癌/细胞周期Key words
RNA interference/ tetraspanin/ hepatocellular carcinoma/ cell cycle分类
医药卫生引用本文复制引用
李坤雨,李牛,王群,张壮壮,韩泽广,邓庆..RNA干扰沉默编码跨膜蛋白35基因表达对肝癌细胞生长及细胞周期的影响[J].兰州大学学报（医学版）,2012,38(3):17-22,26,7.基金项目
上海市科委自然科学基金(11ZR1425300) （11ZR1425300）
国家自然科学基金(81101875) （81101875）
