兰州大学学报（医学版）2013，Vol.39Issue(1)：1-5,5.
三磷酸腺苷对骨髓间充质干细胞促进脊髓损伤后肢体功能恢复的影响
Effect of adenosine triphosphate on the functional recovery of rats spinal cord injury after transplantation of bone marrow-mesenchymal stem cells
摘要
Abstract
Objective To study the effect of adenosine triphosphate (ATP) in aiding the functional recovery in rats spinal cord injury (SCI) after transplantation of bone marrow-mesenchymal stem cells (BMSCs) delivery on lesion site. Methods 48 experimental adult female Sprague dawley rats (weighing 240～260 g) were made SCI models at Ti2 levels according to the improved Allen's method and were divided into 4 groups randomly. Group A, ATP (40 mg/kg) and BMSCs (6 μL) were injected to the SCI; group B, only BMSCs were injected; group C, only ATP was injected; group D, only laminectomy. The general conditions and their motility of hind limb were regularly scored using the improved Tarlov scale on 1, 3, 7, 14, 21 and 28 days after operation. After 4 weeks, rats were sacrificed, and their spinal cords were examined for 5-bromo-2-deoxy uridine labeled BMSCs by immunostainings. Results Paralysis symptom in hind limb occurred in all experimental rats. There was gradual improvement noticed in group A, B and C after the operation and significant motor recovery in group A at 2～3 weeks. No obvious change was observed in group D. The scores, except those between groups B and C, showed significant differences among groups on 14, 21 and 28 days after operation (P <0.05). The ultrastructures of spinal cord tissue showed immunohistochemical positive staining cells with antibody of anti-5-bromo-2-deoxy uridine in groups A and B on 28 days after operation, and the cells of group A was significantly more than those of group B (P <0.05). There were no positive staining cells in groups C and D. Conclusion ATP had the effect of synergistical action on reparation using BMSCs in SCI.关键词
三磷酸腺苷/骨髓间充质干细胞/脊髓损伤/肢体功能Key words
adenosine triphosphate/ bone marrow mesenchymal stem cells/ spinal cord injury/ limb function分类
医药卫生引用本文复制引用
刘文忠,陈永刚,王栓科,董平,马延超..三磷酸腺苷对骨髓间充质干细胞促进脊髓损伤后肢体功能恢复的影响[J].兰州大学学报（医学版）,2013,39(1):1-5,5.基金项目
甘肃省自然科学基金(0803RJZA081) （0803RJZA081）
