环境与职业医学2013，Vol.30Issue(3)：176-179,4.
锰致PC12细胞PARK2表达对多巴胺分泌的修饰作用
Modification of Dopamine Secretion in PC12 Cells by Manganese Induced PARK2 Gene Expression
摘要
Abstract
[ Objective ] To study the effect of manganese on the content of dopamine and the expression of PARK2 in pheochromocytoma (PC12) cells at different doses and different time lengths. [ Methods ] Rat PC12 cells were incubated for 24 h with 0,100, 300, 500 μmol/L of MnCl2, and incubated with 500 μmol/L MnCl2 for 6, 24, and 48 hours. The content of dopamine in PC 12 cells were determined by reverse phase high performance liquid chromatography combined with fluorometric detection. The expression of PARK2 mRNA was examined by real time quantitative polymerase chain reaction. [ Results ] The dopamine content and PARK2 mRNA expression level tended to decline with the increasing MnCl2 concentrations. Compared with the control group, after 24 h of incubation, the 300 and 500 μmol/L MnCh significantly decreased the dopamine content and PARK2 expression (P<0.0l). When the PC12 cells were exposed to 500μmol/L Mncl2 for 6, 24, and 48h respectively, the content of dopamine and the expression of PARK2 mRNA were also significantly lowered (P<0.01, P<0.05 respectively). [ Conclusion ] Manganese could reduce dopamine content in PC 12 cells, and the effect may be associated with down-regulated PARK2 expressions.关键词
锰/PC12细胞/多巴胺/PARK2Key words
manganese/ PC12 cells/ dopamine/ PARK2分类
医药卫生引用本文复制引用
邱静,范磊,陶冬,隋典朋,邓妍,范奇元..锰致PC12细胞PARK2表达对多巴胺分泌的修饰作用[J].环境与职业医学,2013,30(3):176-179,4.基金项目
国家自然科学基金项目(编号:81260420) （编号:81260420）
贵州省科技计划课题[编号:黔科合SY字(2012)3140] （2012）
