林业科技开发2013,Vol.27Issue(2):116-118,3.DOI:10.3969/j.issn.1000-8101.2013.02.031
厚叶岩白菜组织培养技术
Study on tissue culture of Bergenia crassifolia (L.) Fritsch
摘要
Abstract
Tissue culture of Bergenia crassifolia ( L. ) Fritsch was developed by organogenesis with apical buds as the ex-plants. The results were that, the efficient multiplication medium was reformed MS supplement with 0. 1 mg/L NAA, 1.0 mg/L BA, and 0. 5 mg/L PVP, the average multiplication rate was 3.03. 1/2MS with IBA 1.0 mg/L and PVP 0.5 mg/L was the optimal rooting medium, and the rooting rate was about 90%. Acclimatized the regenerated plants in medium of muck, yellow sand and perlite(2:1:1) , the survival rate reached to 90% . Therefore, the rapid propagation system for Bergenia crassifolia was established.关键词
厚叶岩白菜/离体培养/标准化生产Key words
Bergenia crassifolia (L.) Fritsch/ tissue culture/ commercial production引用本文复制引用
吕秀立,沈烈英,王健,王景先..厚叶岩白菜组织培养技术[J].林业科技开发,2013,27(2):116-118,3.基金项目
新疆维吾尔自治区科技支撑项目"厚叶岩白菜引种驯化栽培技术研究与成分分析"(编号:201231109). (编号:201231109)