林业科学2013，Vol.49Issue(3)：63-69,7.DOI:10.11707/j.1001-7488.20130309
杜仲雄花提取物的体外抗氧化活性评价
Evaluation of the Antioxidant Activity of the Extracts from Eucommia Male Flower in vitro
摘要
Abstract
The male flowers of Eucommia ulmoides were extracted by the solvents with different polarities, namely, petroleum ether, ethyl acetate, n-butanol, and water. The contents of total phenols in the obtained extracts were measured, and their antioxidant activities were examined in vitro and compared with those of BHT and VC, which were the two conventional reductants, from the aspects of scavenging rate of DPPH and hydroxyl free radicals, reducing power, and anti-lipid per-oxidation activity, respectively. The results indicated that Eucommia male flower was rich in phenols. The content of phenols in ethyl acetate extract (EE) (393.47 mg·g-1) and ra-butanol extract (BE) (287.33 mg·g-1) were higher than those in water extract (WE) (114.74 mg·g-1) and petroleum extract (PE) (105.42 mg-g-1). The rates of scavenging DPPH free radical of the EE and BE were 91. 55% and 80. 32% , respectively, close to those of BHT and VC. The rate of scavenging hydroxyl free radical of BE was relatively higher (80. 47% ) , while the reducing power of EE was higher than those of BHT and VC. The activities of anti-lipid per-oxidation of EE, BE and WE were higher than that of VC. The reducing power, rate of scavenging DPPH free radical and activity of anti-lipid per-oxidation of the extracts were positively correlated to the content of total phenols ( R2 ＞ 0. 9 ) , and exhibited significant concentration dependent effect. The relatively higher antioxidant activity of Eucommia male flower demonstrated that it could be developed as a health care beverage.关键词
杜仲/杜仲雄花/总酚/抗氧化活性Key words
Eucommia ulmoides/ Eucommia male flower/ total phenol/ antioxidant activity分类
轻工纺织引用本文复制引用
邱高翔,董娟娥,马希汉,行冰玉,张靖一,雷鸣..杜仲雄花提取物的体外抗氧化活性评价[J].林业科学,2013,49(3):63-69,7.基金项目
国家林业公益性项目科研专项(201204603) （201204603）
"十一五"国家科技支撑计划项目(2006BAD18B03). （2006BAD18B03）
