林业科学2013，Vol.49Issue(3)：165-169,5.DOI:10.11707/j.1001-7488.20130323
落叶松毛虫2种解毒酶的体躯分布特征及对3种杀虫剂敏感性比较
Tissue Distribution of Two Detoxifying Enzymes in Dendrolimus superans and Their Susceptibility to Three Pesticides
摘要
Abstract
Distribution of carboxylesterase (CarE) and glutathione-S transferase (GST) in the midgut, fat body, head and epidemis tissues of Dendrolimus superans 6th instar was determined. The results showed that both CarE and GST distributed in all the four tested tissues, however, CarE predominated in midgut (61.36%) while GST in fat body (36. 15% ). The susceptibility of CarE and GST to DDVP, avermectins and lambda-cyhalothrin was also measured in vitro. The three pesticides had different degree inhibition to either CarE or GST. There were distinct dose-effect relationships between each pesticide and each enzyme. DDVP showed the highest inhibitive effect to CarE( IC50 ＜ 1. 000 × 10-4 mg·mL-1 ) , followed by avermectins (IC50 = 1. 259 × 10 -2 mg×mL-1 ) and then lambda-cyhalothrin (IC50 =4. 529 × 10-1 mg·mL-1 ). However, avermectins showed the highest inhibitive effect to GST, followed by lambda-cyhalothrin, DDVP showed the weakest inhibition to GST. The IC50 values were 8. 044 × 10 -3, 9. 224 × 10- 3 and 1. 755 × 10 -2 mg· mL-1 respectively. These results provide important information for reasonable insecticide application to control D. superans.关键词
落叶松毛虫/羧酸酯酶/谷胱甘肽-S转移酶/杀虫剂/敏感性Key words
Dendrolimus superans/ carboxylesterase/ glutathione-S transferase/ pesticide/ sensitivity分类
农业科技引用本文复制引用
邹传山,曹传旺,张国财,王志英..落叶松毛虫2种解毒酶的体躯分布特征及对3种杀虫剂敏感性比较[J].林业科学,2013,49(3):165-169,5.基金项目
国家林业公益性行业科研专项课题(201104069) （201104069）
黑龙江省科技计划项目(GB08B202-02 （）
GA09B203-3) （）
中央高校基本科研业务费专项资金项目(DL11BA17). （DL11BA17）
