国际眼科杂志2013，Vol.13Issue(4)：674-651,5.DOI:10.3980/j.issn.1672-5123.2013.04.01
补肾活血中药对大鼠慢性高眼压模型初级视皮质BDNF损害的影响
Influence of bushenhuoxue on primary visual cortex' BDNF damage in rat model of chronic elevated intraocular pressure
摘要
Abstract
·AIM:To observe the effect of traditional Chinese medicine (TCM)of bushenhuoxue on primary visual cortex (PVC)brain-derived neurotrophic factor (BDNF)in rat model of chronic elevated intraocular pressure (EIOP), and explore the mechanism of it initially.·METHODS:The rat model of chronic EIOP was established by unilaterally cauterizing 3 episcleral veins, then 30 rats were divided into 3 groups randomly:control group, model group, and treatment group. After given drugs or normal saline for 8 weeks, the rats were put to death. The effect of intraocular pressure (IOP), expression of BDNF and ultrastructure of neuron cell in the PVC was observed.·RESULTS:Unilaterally cauterizing episcleral veins increased IOP of the rat model obviously, there was significant difference compared with pre-operation (P<001). Semi-quantitative pathological analysis on PVC showed that BDNF of total area in the model group was (82438±2597.39)S/μm2,mean optical density was (1155.9±123.14), integrated optical density was (12915±673.28), compared with the control group {total area was (132370±7588.47)S/μm2, mean optical density was (5365±379.65), integrated optical density was (35102±2648.5)}, there were statistical differences (all P<0.05)，there was statistical difference in BDNF of total area between model group and treatment group{ (108980±9126.77)S/μm2, P<005}, significant difference in mean optical density between the model group and treatment group(3220.4±413.67, P<0.05), statistical difference in integrated optical density between the model group and treatment group(23821±3431.68, P<0.05).·CONCLUSION:TCM of bushenhuoxue can repair the PVC damage in the rat model of chronic EIOP by enhancing expression of BDNF, improving ultrastructure of neuron cell.关键词
青光眼/大鼠慢性高眼压模型/补肾活血法/初级视皮质/尼氏体/神经元细胞超微结构Key words
glaucoma/ rat model of chronic elevated intraocular pressure/ TCM of bushenhuoxue/ primary visual cortex/ nissl bodies/ ultrastructure of neuron cell引用本文复制引用
李翔,郭红建,文晓霞,谢钊,谢学军,路雪婧,王毅,张静..补肾活血中药对大鼠慢性高眼压模型初级视皮质BDNF损害的影响[J].国际眼科杂志,2013,13(4):674-651,5.基金项目
中国四川省教育厅2010年重点项目(No.10ZA096) （No.10ZA096）
