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大鼠脊髓前角与盆神经节之间植块联合培养的实验研究

程时刚 耿红琼 张伊凡 杨星海 钟伟

南京医科大学学报(自然科学版)2013,Vol.33Issue(2):177-181,5.
南京医科大学学报(自然科学版)2013,Vol.33Issue(2):177-181,5.DOI:10.7655/NYDXBNS20130207

大鼠脊髓前角与盆神经节之间植块联合培养的实验研究

Experimental study on co-culture of ventral spinal cord explants and major pelvic ganglion explants

程时刚 1耿红琼 1张伊凡 1杨星海 1钟伟1

作者信息

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摘要

Abstract

Objective;To co-culture the ventral spinal cord explants and major pelvic ganglion explants from rats and investigate the interaction between co-cultured explants. Methods:We had developed a system for long-term co-culturing of ventral spinal cord explants and major pelvic ganglia explants to observe the variance of neuron growth. Results ;Explanted tissues of both types survived well in co-culture. Spinal motor neurons (SMNs) emitted numerous outgrowing processes, some of which associated with neurons in explanted major pelvic ganglia. Conclusion: Some contacts of specific motor neuron-major pelvic ganglia neuron were observed apparently,suggesting that a functional interaction may develop between the spinal motor neurons and major pelvic ganglion neurons in vitro.

关键词

脊髓前角/盆神经节/植块/联合培养

Key words

ventral spinal cord/major pelvic ganglion/explants/co-culture

分类

生物科学

引用本文复制引用

程时刚,耿红琼,张伊凡,杨星海,钟伟..大鼠脊髓前角与盆神经节之间植块联合培养的实验研究[J].南京医科大学学报(自然科学版),2013,33(2):177-181,5.

南京医科大学学报(自然科学版)

OA北大核心CSCDCSTPCD

1007-4368

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