南京医科大学学报（自然科学版）2013，Vol.33Issue(2)：195-200,6.DOI:10.7655/NYDXBNS20130211
602例血培养阳性病原菌的分布及其耐药性分析
Analysis of the distribution and resistance of 602 pathogens isolated from blood culture
摘要
Abstract
Objective:To investigate the distribution and resistance of positive pathogens isolated from blood culture in the First Affiliated Hospital of Nanjing Medical University in 2011. Methods: Automatic blood culture instrument of BACTEC 9240 was used for blood samples; bacteria and fungus were identified by API system and automatic detection machine of VITEK2;bacterial drug sensitivity was determined by disk diffusion method;drug sensitivity of fungi was determined by Rosco method;and Whonet 5.4 software was used for data analysis. Results:Among the 4 051 blood culture samples,602 cases showed positive.Gram-negative bacteria accounted for 47.5% , gram-positive bacteria for 45.0% , fungi for 7.0% and anaerobes for 0.5%. Of the Gram-positive bacteria,61.8% were Staphylococcus, 18.0% were Enterococcus and 14.3% were Streptococcus. There was no resistance to linezolid, vancomycin and teicoplanin in Staphylococc us, while nine stains were intermediary sensitive to teicoplanin. The sensitive rate of Enterococcus to linezolid was 100.0%, but two stains of E. faecium were resistant to vancomycin or teicoplanin. The Gram-negative bacteria were composed mainly of Escherichia coli,Klbesiella pneumoniae ,Acinetobacter bauamnnii and Pseudomonas aeruginosa. The sensitive rates of Enterobacleriaceae to imipenem and meropenem were 86.8% and 85.8%. Acinetobacter bauamnnii had a relatively high resistance to many antimicrobial drugs,which was above 50.0%. Conclusion;There is a variety of pathogens isolated from the blood culture,which show an increasing trend of the resistance to many antimicrobial agents. Strengthening surveillance allows of no delay.关键词
血培养/病原菌/敏感性/耐药率Key words
blood culture/pathogen/sensitivity/resistance分类
医药卫生引用本文复制引用
张小莉,王珏,陈友华,顾兵,梅亚宁,文怡..602例血培养阳性病原菌的分布及其耐药性分析[J].南京医科大学学报（自然科学版）,2013,33(2):195-200,6.基金项目
国家自然基金项目(81000754) （81000754）
江苏省实验诊断学重点实验室重大课题(XK201114) （XK201114）
