热带作物学报2013，Vol.34Issue(2)：272-275,4.DOI:10.3969/j.issn.1000-2561.2013.02.014
橡胶草胶乳蛋白双向电泳方法的建立
Establishment of Two-dimensional Electrophoresis for Taraxacum Kok-saghyz Rodin Latex Protein
摘要
Abstract
The total protein samples of Taraxacum Kok-saghyz Rodin (TKS) latex extracted by Tris-saturated phenol method and trichloroacetic acid (TCA) /acetone precipitation method were analyzed by two-dimensional electrophoresis ( 2 -DE). The 2 -DE gel of TCA/acetone precipitation showed strong horizontal streaks and background blur, and 446 protein spots; While the Tris-saturated phenol extraction was capable of generating more than 630 protein spots, obtaining better effect of isoelectric focusing. There were more small proteins revealed by Tris-saturated phenol extraction.关键词
橡胶草/胶乳/蛋白质/电泳Key words
Taraxacum Kok-saghyz Rodin/ Latex/ Protein/ Electrophoresis分类
农业科技引用本文复制引用
黄荣辉,校现周,仇键,刘实忠,罗世巧,王启超,梅志刚,牛静明..橡胶草胶乳蛋白双向电泳方法的建立[J].热带作物学报,2013,34(2):272-275,4.基金项目
乙烯刺激对橡胶树萜类次生代谢的调控(No.XJSYWFZX2009-15). （No.XJSYWFZX2009-15）
