热带作物学报2013，Vol.34Issue(2)：285-291,7.DOI:10.3969/j.issn.1000-2561.2013.02.016
一个新的水稻亚精胺合成酶基因克隆、表达及特性分析
Molecular Cloning, Expression Patterns and Characterization of a New Spermidine Synthase Gene in Oryza sativa
摘要
Abstract
Spermidine synthase is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethionine to putrescine in the biosynthesis of spermidine,and which plays a critical role in a wide range of growth and developmental process.In this research,a new spermidine synthase gene,designated as OsSPDS3,was isolated by 3‘ RACE method in Oryza sativa.The OsSPDS3 contained an 1194 bp open reading frame encoding 397 amino acids.The OsSPDS3 included a complete spermine_synth domain,two putative low complexity regions and several binding sites of S-adenosylmethionine.The predicted 3-D structure indicated that OsSPDS3 contained α-helices,β-sheets and coils.The phylogenetic analyses revealed that OsSPDS3 belonged to plant spermidine synthase family,in which AtSODS3,OsSPDS3 and OsSPDS2 were clustered into one clade.OsSPDS3 transcripts were significantly up-regulated under high salinity stress.In contrast,no such induction of the OsSPDS1 and OsSPDS2 were observed under high salinity stress.In addition,the expressions of three rice spermidine synthase genes did not change under drought and cold treatments.The results suggested that OsSPDS3 indicated a distinct function in high salinity stress.关键词
非生物逆境/OsSPDS3/水稻/亚精胺合成酶Key words
Abiotic stress/ OsSPDS3/ Oryza sativa/ Spermidine synthase分类
生物科学引用本文复制引用
郭会娜,夏志辉,李德军..一个新的水稻亚精胺合成酶基因克隆、表达及特性分析[J].热带作物学报,2013,34(2):285-291,7.基金项目
国家自然科学基金项目(No.31071173) （No.31071173）
教育部重点项目(No.210173). （No.210173）
