水产学报2013，Vol.37Issue(4)：593-598,6.DOI:10.3724/SP.J.1231.2013.38304
黄喉拟水龟巨噬细胞炎症蛋白-3α重组表达及抗菌活性分析
Recombinant expression and antimicrobial activity analysis of macrophage inflammatory protein-3 α in Mauremys mutica
摘要
Abstract
Macrophage inflammatory protein-3a is a kind of inducible secreted protein which plays an important role in the immune response and inflammation damage in many diseases through the corresponding receptors on a variety of immune cells to produce chemokines. We obtained the ORF of MaCCL20 by RT PCR,inserted into the expression vector pET-32a,and then transformed into E. coli BL21. We expressed the fusion protein pET-32a-CCL20 after IPTG induction,prepared the polyclonal antibodies and did research on Western blot and antimicrobial activity of it. The experiments show that the fusion protein is highly expressed in the conditions of 37 t ,0. 8 mmol/L IPTG for 4 hours. The SDS-PAGE gel electrophoresis figure shows that the relative molecular weight of the fusion protein is 30 ku which is the same as predicted before. We got a single band after His Bind nickel column purification indicating the high purity of MaCCL20 recombinant protein. The antibacterial experiments show that MaCCL20 recombinant protein has strong antibacterial effects on Staphylococcus aureus ,Serratia marcescens, and Aeromonas hydrophila.关键词
黄喉拟水龟/巨噬细胞炎症蛋白-3α/重组表达/抗菌活性Key words
Mauremys mutica/macrophage inflammatory protein-3a/recombinant expression/antibacterial activity分类
农业科技引用本文复制引用
龙建杰,朱新平,史燕,赵建,洪孝友..黄喉拟水龟巨噬细胞炎症蛋白-3α重组表达及抗菌活性分析[J].水产学报,2013,37(4):593-598,6.基金项目
国家农业科技成果转化资金项目(2011GB23260021) （2011GB23260021）
中国水产科学研究院院级基本科研业务费专项(2012A0403) （2012A0403）
广州市珠江科技新星专项(2012089) （2012089）
