山东医药2013，Vol.53Issue(6)：12-14,3.DOI:10.3969/j.issn.1002-266X.2013.06.004
MCF-7/PTX多药耐药细胞株中CD44+CD24-乳腺癌干细胞富集的研究
Research on the enrichment of CD44+CD24-cancer stem cells in breast cancer multi-drug resistant cell lines MCF-7/PTX
摘要
Abstract
Objective To establish human breast cancer multi-drug resistant cell lines MCF-7/PTX in order to study the enrichment of CD+44CD-24 breast cancer cells. Methods We established the multidrug resistant cell line MCF-7/PTX by method of intermittently induction with high dose of PTX. The resistance and cross-resistance of MCF-7 and MCF-7/PTX on PTX were identified by MTT method. The proportion of CD+44CD-24 cells was detected by method of flow cytometry. Results The resistance indices of MCF-7/PTX on paclitaxel, doxorubicin, docetaxel, epirubicin, vincristine, methotrexate, cis-platin were 53, 45.72, 38.30,41.26, 23.75, 17.62 and 35. 80, respectively. The proportion of CD+44CD-24 cells in MCF-7 and MCF-7/PTX were 8.60% , 73. 20% , respectively. Conclusion The MCF-7/PTX cell line established in this study was a kind of multi-drug resistant cell line. The CD+44CD-24 cells in MCF-7/PTX were enriched.关键词
肿瘤干细胞/多药耐药/紫杉醇多药耐药细胞株/乳腺癌Key words
cancer stem cells/multidrug resistance/MCF-7/PTX/breast cancer分类
医药卫生引用本文复制引用
韩娜娜,孙长岗,庄静,杨静..MCF-7/PTX多药耐药细胞株中CD44+CD24-乳腺癌干细胞富集的研究[J].山东医药,2013,53(6):12-14,3.基金项目
国家自然科学基金资助项目(99982679-6106-0). （99982679-6106-0）
