山东医药2013，Vol.53Issue(6)：29-32,4.DOI:10.3969/j.issn.1002-266X.2013.06.009
鲤鱼汤对阿霉素肾病大鼠肾脏MMP-2和TIMP-2表达的影响
Effect of cyprinus carpio decoction on the expression of MMP-2 and TIMP-2 in kidney of rats with adriamycin-induced nephropathy
摘要
Abstract
Objective To investigate the mechanism of cyprinus carpio decoction on adriamycin nephrosis. Methods A total of 40 male Wistar rats were involved in this study, of which 10 rats were divided into control group and the other 30 rats were injected by doxorubicin in a dose of 6.2 mg/kg through tail vein to induce adriamycin nephrosis model. The 30 adriamycin nephrosis model rats were then divided equally into 3 group which was named as model group, irbesartan group and cyprinus carpio decoction group. Rats in cyprinus carpio decoction group were intragastric administrated with 300% cyprinus carpio decoction in a dose of 9 mL/kg perday. Rats in irbesartan group were intragastric administrated with irbesartan in a dose of 50 mL/kg perday. Rats in model group and control group were intragastric administrated with water in a dose of 9 mL/kg perday. Urine in 12 hours of each group was collected weekly to measure urinary protein. All rats were killed after the intervening of 8 weeks to observe their pathological changes of kidney, detect TP, ALB, BUN, Cr and detect the expression of MMP-2 and TIMP-2 in kidney by method of immunohistochemistry. Results Compare with control group, the levels of MMP-2 and TIMP-2 of model group decreased, BUN and Cr increased, TP and ALB decreased(P < 0.05), as well as interstitial fibrosis appeared, glomerular volume increased. Compared with model group, the levels of MMP-2 and TIMP-2 in cyprinus carpio decoction group increased, Cr and BUN decreased, TP and ALB increased ( P < 0.05). Conclusion Cyprinus carpio decoction was positive in the reduction of urinary albumin excretion rate, remission of glomerular extracellular matrix accumulate and renal fibrosis. The mechanism may be associated with the regulate balance of MMP-2/TIMP-2.关键词
鲤鱼汤/阿霉素肾病/基质金属蛋白酶-2/基质金属蛋白酶抑制因子-2Key words
cyprinus carpio decoction/adriamycin nephropathy/MMP-2/TIMP-2分类
医药卫生引用本文复制引用
高英,齐玉梅,沈卫,郭云良,刘宗宝,张宇,肖慧娟..鲤鱼汤对阿霉素肾病大鼠肾脏MMP-2和TIMP-2表达的影响[J].山东医药,2013,53(6):29-32,4.基金项目
国家自然科学基金资助项目(81072754). （81072754）
