山东医药2013，Vol.53Issue(6)：33-36,4.DOI:10.3969/j.issn.1002-266X.2013.06.010
EGCG对糖尿病肾病模型大鼠肾小管间质性损害的治疗作用及机制
Influence of EGCG on tubulointerstitial damage in diabetic nephropathy rats
摘要
Abstract
Objective To investigate the effects of (-)-Epigallocatechin 3-0-Gallate ( EGCG) on tubulointerstitial damage and fibrotic correlation factors in diabetic nephropathy rats. Methods Sixty male wistar rats were fed by high-glucose diet for four weeks. All of them were randomly divided into normal control group (A group, n = 16) and diabetic model group (n=44). In the fourth weekend, STZ (30 mg/ kg) was injected into abdominal cavity of rats in diabetic model group. After 48 hours, blood glucose was tested. Thirty nine rats of diabetic model group (survival, qualified and normal appetite) were randomly divided into diabetic nephropathy model group (B group, n = 19) and model treatment group (C group) (n = 18). Rats in A, B and C groups were given high-protein diet and each rats in C group was added EGCG 1g/ d. At 12th weekend, fasting plasma glucose, HbAlc, SCr, BUN, 24 h urinary protein and urinary β2-MG were measured. The levels of AGEs and TGF-β1 in serum were determined by fluorospectrophotometry and ELJSA. The renal pathological changes were examined and the expressions of TGF-β1, VEGF and RAGE in tubulointerstitial were detected by immunohis-tochemical method and immunofluorescence. Results There were significantly difference among A, B, and C group in fasting plasma glucose, HbAlc, SCr, BUN, 24 h urinary protein and urinary β2-MG, AGEs and TGF-β. Fasting plasma glucose, HbAlc, SCr, BUN, 24 h urinary protein and urinary β2-MG, AGEs and TGF-β were significantly higher in B group than those in A group and C group(P <0.05). Renal pathology showed that tubulointerstitial lesion in C group was lessened than B group and expressions of TGF-β1 ,VEGF and RAGE in tubulointerstitial of C group were reduced than B group. Conclusion EGCG could lessen tubulointerstitial damage of diabetic nephropathy in rats and down-regulate the express of fibrotic correlation factors. The renoprotective effects may be related to its inhibition on oxidative stress.关键词
(-)-Epigallocatechin 3-O-Gallate/糖尿病肾病/肾小管/氧化应激Key words
EGCG/diabetic nephropathy/tubulointerstitial/oxidative stress分类
医药卫生引用本文复制引用
马祖等..EGCG对糖尿病肾病模型大鼠肾小管间质性损害的治疗作用及机制[J].山东医药,2013,53(6):33-36,4.基金项目
广东省中医药局计划项目(20110230) （20110230）
深圳市科技局计划项目(201103238). （201103238）
