山东医药2013，Vol.53Issue(8)：22-25,4.DOI:10.3969/j.issn.1002-266X.2013.08.007
线粒体KATP开放剂二氮嗪对家兔心肌缺血—再灌注损伤的影响
Effects of mitochondrial ATP-sensitive potassium channel opener diazoxide on myocardial ischemia-reperfusion injury in rabbits
摘要
Abstract
Objective To investigate the effects of mitochondrial ATP-sensitive potassium channel opener diazoxide on myocardial ischemia-reperfusion (I/R) injury in rabbits. Methods The rabbits were randomly divided into A, B, C, D group with 8 each, respectively, in before making the model 30 min through ear vein giving diazoxide solvent and 1,3,5 mg/kg diazoxide. The rabbit myocardial I/R injury model was established by ligating left anterior descending branch of coronary artery for 30 min and reperfusing for 120 min. During I/R period, the blood pressure, heart rates and electrocardiograms were recorded and the serum creatine kinase (CK) levels were measured. The myocardial infarct sizes were calculated by computer image analysis system. Results Compared with the A group, B and C groups had no significant effects on blood pressure and heart rates. But D group decreased the blood pressure remarkably (P＜0. 01). A, B, C, D groups of myocardial infarction size of the left ventricular area percentage were 22. 7% ±9. 2% , 19. 1% ±5. 8% , 12. 7% ±4. 5% and 11. 8% ± 7. 2% . Myocardial infarction was lowered by 44. 1 % and 48. 0% in C and D groups respectively compared to A group (P ＜0. 05). And C group decreased serum CK levels by 16. 3% after 1 h of reperfusion and 25. 8% after 2 h of reperfusion, while D group decreased by 31.8% and 45.6% respectively (P＜0.01). Conclusion Mitochondrial ATP-sensitive potassium channel opener diazoxide has a myocardial protective effect on myocardial I/R injury in rabbits.关键词
线粒体/ATP敏感性钾通道/二氮嗪/心肌缺血再灌注损伤/家兔Key words
mitochondria/ ATP-sensitive potassium channel/ diazoxide/ myocardial ischemia-reperfusion injury/ rabbit分类
医药卫生引用本文复制引用
刘蔚,李莹莹,汪海..线粒体KATP开放剂二氮嗪对家兔心肌缺血—再灌注损伤的影响[J].山东医药,2013,53(8):22-25,4.基金项目
军队十五规划科研基金重点项目(01Z025). （01Z025）
