实验动物与比较医学2013，Vol.33Issue(1)：7-11,5.DOI:10.3969/j.issn.1674-5817.2013.01.002
红海海绵素A对小鼠精子冷冻活力的影响
Effect of Latrunculin A on Motility of Spermatozoa Cryopreservation in ICR Mouse
摘要
Abstract
Objective To evaluate the effect of latrunculin A on motility of spermatozoa combined with optimized cooling rate of cryopreservation. Methods ICR mouse epididymal spermatozoa balanced in freezing medium were frozen at fast (-240℃/min), medium (-109℃/min), slow (-41 ℃/min) and super slow (-12℃/min) cooling rate for 5 min or 10 min respectively, before being plunged into liquid nitrogen for storage. Results No difference of sperm motility was observed between spermatozoa frozen for 5 and 10 min above liquid nitrogen at each cooling rate. Spermatozoa cryopreserved at super slow and medium cooling rate showed highest and lowest post-thaw motility respectively. ICR mouse spermatozoa were treated with 0.5 μmol/L, 1 umol/L, 2 umol/L and 4 umol/L Latrunculin A before freezing and then cryopreserved at super slow cooling rate. The result showed that treating mouse spermatozoa with 0.5 umol/L, 1 umol/L and 2 umol/L of Latrunculin A could not improve the motility of spermatozoa. However, high level (4 umol/L) of Latrunculin A showed detrimental effect on ICR mouse sperm motility. Conclusion Cryopreservation proficiency of Latrunculin A on sperm is open to discussion.关键词
小鼠精子/冷冻降温速率/红海海绵素A/活力Key words
Mouse spermatozoa/ Cooling rate/ Latrunculin A/ Motility分类
生物科学引用本文复制引用
程芙蓉,杨世华..红海海绵素A对小鼠精子冷冻活力的影响[J].实验动物与比较医学,2013,33(1):7-11,5.基金项目
国家自然科学基金项目(31071279) （31071279）
云南省科技创新人才计划项目(2011CI009) （2011CI009）
