实验动物与比较医学2013，Vol.33Issue(1)：60-63,68,5.DOI:10.3969/j.issn.1674-5817.2013.01.013
survivin mRNA在辐射诱发的胸腺细胞凋亡中的表达
The Expression of Survivin mRNA on Thymus Tissue Apoptosis Induced Radiation
摘要
Abstract
Objective To observe the expression of survivin mRNA on thymus tissue apoptosis induced by ionizing radiation and study its role in radioactive thymus tissue apoptosis. Methods The mouse were irradiated with the X rays (1.75 Gy per time, 1 time per week for consecutive four times, total doses of 7 Gy).The mouse were killed and the thymus tissue were taken out at 30 and 60 d after they were irradiated(six mouse per time). The cell cycle and apoptosis rate were detected by flow cytometry. Survivin gene mRNA expression was detected by RT-PCR. Results The thymocytes and the of cells in G0/G1 and S phase in irradiation group were similar to that in control group, but the G2/M phase cells percentage was lower after irradiated 30 d and higher after 60 d compared with the control group (P<0.05). The thymocyte apoptosis in irradiation group was higher than that in control and the expression of survivin mRNA reduced significantly (P<0.05) at respectively time point. Results Ionizing radiation can promote apoptosis in thymus by reducing survivin mRNA expressions.关键词
电离辐射/胸腺/凋亡/survivinKey words
Ionizing radiation/ TJiymus/ Apoptosis/ Survivin分类
医药卫生引用本文复制引用
屈景凯,王洪艳,齐亚莉,陈玉丙,宋祥福,龚守良..survivin mRNA在辐射诱发的胸腺细胞凋亡中的表达[J].实验动物与比较医学,2013,33(1):60-63,68,5.基金项目
吉林省科技厅项目资助课题(20080446) （20080446）
