护理研究2013，Vol.27Issue(7)：665-667,3.DOI:10.3969/j.issn.1009-6493.2013.07.047
凋亡因子capase-12在压疮早期损伤中的作用
Effect of apoptotic factor caspase-12 in early injury of pressure ulcers patients
摘要
Abstract
Objective:To analyze the expressions and dynamic change of apoptotic factor caspase - 12 in early injury of pressure ulcers patients, and probe into the molecular mechanism of pressure ulcers,so as to provide theory evidences for prevention of clinical pressure ulcer. Methods: A total of 40 male SD rats were divided into 5 groups randomly, eight rats in each group,control group rats without applying pressure,the experimental group rats simulated clinical pressure ulcer conditions, exerting pressure for 2 h,relax for 0. 5 h,3 cycles for every day. According to different pressure cycles the experimental group was divided into lc,3c, 6c,9c group. By using HE staining to observe tissues pathological changes and immunohistochemical method to detect the dynamic changes of endoplasmic reticulum - specific apoptotic factors caspase - 12 in pressured muscle tissue. Results: Compared with the control group, the results under the light microscope of HE staining showed,the compression muscle tissue of the experiment group showed a gradual degradation in the pathological changes with the extended cycle. The immunohistochemical results indicated that the protein expression of caspase - 12 was elevated in the experiment group compared with the control group (P< 0.05). Conclusion;Early injury of pressure ulcers activated endoplasmic reticulum stress response and activate the apoptotic factors caspase - 12 and then to induce cell damage.关键词
压疮/内质网应激/caspase-12/组织损伤Key words
pressure ulcer/ endoplasmic reticulum stress/ caspase - 12/ tissue injury分类
医药卫生引用本文复制引用
崔飞飞,代彦丽,张龙,潘莹莹,姜丽萍..凋亡因子capase-12在压疮早期损伤中的作用[J].护理研究,2013,27(7):665-667,3.基金项目
温州医学院科研课题,编号:Z 201122386. （）
