山西农业大学学报（自然科学版）2012，Vol.32Issue(6)：540-543,4.
绵羊卵巢卵泡颗粒细胞体外培养中促卵泡素和胰岛素浓度优化研究
Concentration Optimization of Follicle-Stimulating Hormone and Insulin on in Vitro Cultured Ovarian Follicular Granulosa Cells in Sheep
摘要
Abstract
To study more effects of follicle-stimulating hormone (FSH) and insulin on sheep ovarian granulosa cells in serum-free culture in vitro and obtain optimal culture system, we used six different concentrations of FSH and three of insulin. Cell number and E2 concentration were calculated after 7 days. The results indicated that groups treated with FSH or insulin showed a notable increment in stimulating granulosa cell proliferation and estradiol production than control groups (P＜0. 01). Granulosa cells and estradiol production were more, accompanying the higher concentrations of two additives. E2 concentration was the highest, when FSH was 5 μg·L-1. The most granulosa cells and the highest E2 concentration occurred when insulin was 10 μg·L-1. We obtained the best culture concentrations of FSH and insulin in vitro.关键词
FSH/胰岛素/卵巢/颗粒细胞/绵羊Key words
FSH/ Insulin/ Ovary/ Granulosa cell/ Sheep分类
农业科技引用本文复制引用
庞钰莹,岳文斌,于雪静,李鹏飞,孟金柱,刘岩,李晓明,黄洋,任有蛇..绵羊卵巢卵泡颗粒细胞体外培养中促卵泡素和胰岛素浓度优化研究[J].山西农业大学学报（自然科学版）,2012,32(6):540-543,4.基金项目
国家自然基金(31172211) （31172211）
农业部948项目(2010-Z43) （2010-Z43）
山西省留学基金和引进人才基金项目(2008080) （2008080）
山西省国际科技合作项目(2010081002) （2010081002）
国家重点实验室开放课题(SNKF-2012-02) （SNKF-2012-02）
山西农业大学横向协作与委托项目(2010HX54) （2010HX54）
