陕西师范大学学报（自然科学版）2013，Vol.41Issue(2)：70-75,6.
丹参法呢基焦磷酸合酶基因(SmFPPS1)的表达模式
Expression pattern of farnesyl diphosphate synthase (SmFPPS1) from Salvia Miltiorrhiza Bunge
摘要
Abstract
Tanshinones are the major active components of Danshen, which are derived from terpenoids, and farnesyl diphosphate synthase (FPPS) is a key enzyme in terpenoids biosynthetic pathway. A novel FPPS (SmFPPS1, HQ687768) in Salvia miltiorrhiza was cloned and characterized. Bioinformatics analysis showed that SmFPPSl locates in cytoplasm, and it contains a prenyltransferase domain and two Asp-rich characteristic motifs. The functional complementation tests showed that SmFPPSl didn't exhibit activity of GGPPS. Quantitative realtime PCR analysis revealed that the gene expressed constitutively in different development periods, mainly in flowers, and the expression could be induced by methyl jasmonate (MeJA) and fungal elicitor signal.关键词
丹参/法呢基焦磷酸合酶/表达模式Key words
Salvia miltiorrhiza / farnesyl diphosphate synthase/ expression pattern分类
农业科技引用本文复制引用
周露,化文平,杨滢,王喆之,李翠芹..丹参法呢基焦磷酸合酶基因(SmFPPS1)的表达模式[J].陕西师范大学学报（自然科学版）,2013,41(2):70-75,6.基金项目
国家自然科学基金资助项目(31000134) （31000134）
中央高校基本科研业务费重点项目(GK200901014). （GK200901014）
